top of page
A Little More on Genome Sequencing
How Does It Work?
Genome sequencing is analyzing a section of DNA, determining order of bases, and then writing it down. One specific way is using dDNA (di-Deoxy-ribonucleic Acid), which is a nucleotide that does not have OH- ions that are found on regular DNA bases. dDNA are found on the ends of a DNA chain, ending any growth and therefore making it easier to see the sequence. H.4
A Closer Look
To be able to sequence a piece of DNA, you only need to look at one strand, but it has to be separated from the proteins and the growth of the DNA has to be stopped. After the DNA is "clean", it is run through gel electrophoresis, which separates the DNA so it is easier to read.
Challenges
This includes the scientist’s ability to check for accurate information: with millions of nucleotide bases to be processed, there is high risk of mistakes occurring. There is also a cost and time issue when it comes to sequencing,
especially in developing countries due to lack of money, professionals, and facilities to carry out genome sequencing. Finally, there is the nature of certain organisms. For instance, the banana's complexity and polyploidy nature (having many nuclei), makes sequencing difficult for scientists, also increasing the chance for mistakes.
H.6 , H.7, H.8, H.9
First Introduction to the World
Although the process is relatively new, there have been discoveries
throughout history that have led up to genome sequencing. The start of genome sequencing could be traced back to 1950 with the discovery of base pairing nucleotides. H.5
To see more dates on genome sequencing, click here to jump to "Timeline".
bottom of page